Fatty acid analysis of triacylglycerols: Preparation of fatty acid methyl esters for gas chromatography

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• 作者：Ken'ichi Ichihara^a ; ^{ichihara@kpu.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Chihiro Kohsaka^b ; Naohiro Tomari^b ; Tamami Kiyono^b ; Jun Wada^b ; Kiyoo Hirooka^b ; Yoshihiro Yamamoto^b
• 关键词：Fatty acid analysis ; Fatty acid methyl ester ; Triacylglycerol ; Gas chromatography
• 刊名：Analytical Biochemistry
• 出版年：2016
• 出版时间：15 February 2016
• 年：2016
• 卷：495
• 期：Complete
• 页码：6-8
• 全文大小：378 K

文摘

A method to prepare fatty acid methyl esters was developed for fatty acid analysis of triacylglycerols by gas chromatography (GC). Triacylglycerols were mixed with methanolic CH₃ONa in hexane containing a mid-polar solvent for 10 s at room temperature. Under these conditions, trioleoylglycerol was converted to methyl oleate with an average yield of 99.3%. This procedure gave reliable and reproducible data on fatty acid compositions determined by GC.