Structure-based identification of functional residues in the nucleoside-2′-O-methylase domain of Bluetongue virus VP4 capping enzyme
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- 作者：Meredith E. Stewart ; Polly Roy ; polly.roy@lshtm.ac.uk" class="auth_mail" title="E-mail the corresponding author
- 关键词：BTV ; Bluetongue virus ; 2&prime ; -O MTase ; nucleoside-2&prime ; -O-methyltransferase ; 2&prime ; -O MT ; nucleoside-2&prime ; -O-methyltransferase domain ; GTase ; guanylyltransferase ; N7MTase ; guanine-N7-methyltransferase ; m7G ; 7-methylguanosine ; m7 ; methyl group associated with m7G ; PC ; polymerase complex ; BSR4 ; BHK-21 sub-clone expressing VP4 ; AdoMet ; S-adenosyl methionine ; VSV ; vesicular stomatitis virus ; WNV ; West Nile virus ; JEV ; Japanese encephalitis virus
- 刊名：FEBS Open Bio
- 出版年：2015
- 出版时间：2015
- 年：2015
- 卷：5
- 期：Complete
- 页码：138-146
- 全文大小：1691 K
文摘
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The VP4 capping enzyme 2′-O MTase activity is independent of upstream functions.
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A single mutation within the catalytic tetrad K-D-K-E abolishes 2’-O MTase activity.
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2′-O MTase activity is critical for virus replication.
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Mutations at cap0 binding pocket affect 2′-O MTase activity and virus replication.