Development, synthesis and structure-activity-relationships of inhibitors of the macrophage infectivity potentiator (Mip) proteins of Legionella pneumophila and Burkholderia pseudomallei

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• 作者：Florian Seufert^a ; Maximilian Kuhn^a ; Michael Hein^a ; Matthias Weiwad^b ; Mirella Vivoli^c ; Isobel H. Norville^d ; Mitali Sarkar-Tyson^d ; ^e ; Laura E. Marshall^d ; Kristian Schweimer^f ; Heike Bruhn^g ; Paul Rö ; sch^f ; Nicholas J. Harmer^c ; Christoph A. Sotriffer^a ; Ulrike Holzgrabe^a ; ^{ulrike.holzgrabe@uni-wuerzburg.de" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Mip ; macrophage infectivity potentiator ; PPIase ; peptidyl-prolyl cis/trans isomerase ; LpMip ; L. pneumophila Mip ; BpMip ; B. pseudomallei Mip ; SAR ; structure&ndash ; activity relationship ; HSQC ; Heteronuclear Single Quantum Coherence ; NMM ; N-methylmorpholine ; DIPEA ; N ; N-diisopropylethylamine ; EDC· ; HCl ; 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride ; DMAP ; dimethylaminopyridine ; HOBt ; 1-hydroxybenzotriazol ; HEPES ; 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid
• 刊名：Bioorganic & Medicinal Chemistry
• 出版年：2016
• 出版时间：1 November 2016
• 年：2016
• 卷：24
• 期：21
• 页码：5134-5147
• 全文大小：1368 K

文摘

The bacteria Burkholderia pseudomallei and Legionella pneumophila cause severe diseases like melioidosis and Legionnaire’s disease with high mortality rates despite antibiotic treatment. Due to increasing antibiotic resistances against these and other Gram-negative bacteria, alternative therapeutical strategies are in urgent demand. As a virulence factor, the macrophage infectivity potentiator (Mip) protein constitutes an attractive target. The Mip proteins of B. pseudomallei and L. pneumophila exhibit peptidyl-prolyl cis/trans isomerase (PPIase) activity and belong to the PPIase superfamily. In previous studies, the pipecolic acid moiety proved to be a valuable scaffold for inhibiting this PPIase activity. Thus, a library of pipecolic acid derivatives was established guided by structural information and computational analyses of the binding site and possible binding modes. Stability and toxicity considerations were taken into account in iterative extensions of the library. Synthesis and evaluation of the compounds in PPIase assays resulted in highly active inhibitors. The activities can be interpreted in terms of a common binding mode obtained by docking calculations.