• 作者：Joshua D. Tompkins^a ; ^e ; ^{jtompkins@coh.org" class="auth_mail" title="E-mail the corresponding author} ; Marc Jung^a ; ^e ; Chang-yi Chen^b ; ^c ; ^e ; Ziguang Lin^b ; ^c ; ^e ; Jingjing Ye^b ; ^c ; ^e ; Swetha Godatha^a ; ^e ; Elizabeth Lizhar^a ; ^e ; Xiwei Wu^d ; ^e ; David Hsu^b ; ^c ; ^e ; Larry A. Couture^b ; ^c ; ^e ; Arthur D. Riggs^a ; ^e
• 关键词：Human embryonic stem cells ; Pluripotent ; Cardiomyocytes ; Differentiation ; Cardiomyogenesis ; DNA methylation ; Long non-coding RNA ; lncRNA ; Epigenetic ; Methylome ; Transcriptome ; Mesoderm ; Good manufacturing practice ; GMP ; epigenetic memory ; WNT ; hedgehog ; transforming growth factor ; ROR2 ; PDGFRα ; demethylation ; TET ; TDG ; HOX ; TBOX
• 刊名：EBioMedicine
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：4
• 期：Complete
• 页码：74-85
• 全文大小：2605 K

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Pluripotent-derived-cardiomyocytes from scalable and defined cultures were used for transcriptome and methylome studies.

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Mesoderm cell purification was conducted with ROR2 and PDGFRα surface markers removing heterogeneous cell contaminants.

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Residual differentiation induced exon DNA methylation constitutes a developmental “memory” of transcription

Human embryonic stem cells (hESCs) represent a theoretically unlimited cell replacement source in cardiac regenerative medicine. In this report, Tompkins et al. describe the derivation, differentiation stage-specific purification, and genome-wide analysis of cardiomyocytes derived from hESCs. Key features of the molecular programs that define human cardiac muscle cell differentiation are characterized and researchers observed that cells may harbor epigenetic DNA methylation “memories” that reflect the gene activation history of important developmental genes.