Effects of hyperhomocysteinemia and betaine-homocysteine S-methyltransferase inhibition on hepatocyte metabolites and the proteome

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• 作者：Irena Selicharov¨¢ ; Marek Ko?¨ªnek ; Zuzana Demianov¨¢ ; Martina Chrudinov¨¢ ; Jana Ml¨¢dkov¨¢ ; Ji?¨ª Jir¨¢?ek
• 关键词：2DE ; two-dimensional electrophoresis ; Apo A1 ; apolipoprotein A1 ; BHMT ; betaine&ndash ; homocysteine S-methyltransferase ; CBHcy ; S-(¦Ä-Carboxybutyl)-dl-homocysteine ; Cysta ; cystathionine ; CBS ; cystathionine ¦Â-synthase ; DMG ; dimethylglycine ; Fib-¦Ã ; fibrinog
• 刊名：Biochimica et Biophysica Acta - Proteins and Proteomics
• 出版年：2013
• 出版时间：August, 2013
• 年：2013
• 卷：1834
• 期：8
• 页码：1596-1606
• 全文大小：1838 K

文摘

Both cardiovascular disease and liver injury are major public health issues. Hyperhomocysteinemia has been linked to cardiovascular diseases, and defects in methyl group metabolism, often resulting in hyperhomocysteinemia, are among the key molecular events postulated to play a role in liver injury. We employed proteomics and metabolomics analyses of human hepatocytes in primary cell culture to explore the spectrum of proteins and associated metabolites affected by the disruption of methyl group metabolism. We treated the hepatocytes with homocysteine (Hcy, 0.1 mM and 2 mM) to follow the impact of hyperhomocysteinemia, and in parallel, we used a specific inhibitor of betaine-homocysteine S-methyltransferase (BHMT) to extend our understanding of the physiological functions of the enzyme. The major effect of BHMT inhibition was a 50 % decrease in S-adenosylmethionine levels. The treatments with Hcy resulted in multiple changes in the metabolite levels depending on the treatment modality. The BHMT inhibition and 0.1 mM Hcy treatment induced only moderate changes in the hepatocyte proteome and secretome, while the changes induced by the 2 mM Hcy treatment were extensive. Phosphatidylethanolamine carboxykinase and ornithine aminotransferase were up-regulated about two fold indicating an intervention into metabolism. Cellular proliferation was suspended, secretome composition was changed and signs of apoptosis were discernible. We have detected fibrinogen gamma dimers, which might have a role as a potentially new biomarker of early liver injury. Finally, we have demonstrated the failed maturation of apolipoprotein A1, which might be a new mechanism of disruption of cholesterol efflux from tissues.