Cloning and expressing a highly functional and substrate specific farnesoic acid o-methyltransferase from the Asian citrus psyllid (Diaphorina citri Kuwayama)
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- 作者：Evelien Van Ekerta ; evelienvanekert@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Robert G. Shatters Jr.a ; Robert.shatters@ARS.USDA.GOV" class="auth_mail" title="E-mail the corresponding author ; Pierre Rougé ; b ; pierre.rouge@free.fr" class="auth_mail" title="E-mail the corresponding author ; Charles A. Powellc ; capowell@ufl.edu" class="auth_mail" title="E-mail the corresponding author ; Guy Smagghed ; guy.smagghe@ugent.be" class="auth_mail" title="E-mail the corresponding author ; Dov Borovskya ; dovborovsky@gmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：FA ; farnesoic acid ; FA-o-MT ; farnesoic acid o-methyltransferase ; fmtD ; Diaphorina citri farnesoic acid o-methyltransferase gene ; GAPDH ; glyceraldehyde 3-phosphate dehydrogenase ; JH ; juvenile hormone ; JHA ; juvenile hormone acid ; JHAMT ; juvenile hormone acid methyltransferase ; jmtD ; juvenile hormone acid methyl transferase ortholog gene of Diaphorina citri ; MF ; methyl farnesoate ; MMLV ; Moloney murine leukemia virus ; RP ; reversed phase ; SAM ; S-adenosyl methionine ; SAM-MT ; S-adenosyl methionine-depe
- 刊名：FEBS Open Bio
- 出版年：2015
- 出版时间：2015
- 年：2015
- 卷：5
- 期：Complete
- 页码：264-275
- 全文大小：2039 K
文摘
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A putative JH acid methyltransferase ortholog gene (jmtD) and its cognate cDNA were identified in D. citri.
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This gene is expressed in all life stages in D. citri.
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The purified enzyme expressed from cDNA in E. coli showed high preference for farnesoic acid (FA) and homoFA.
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This suggests that this ortholog is a DcFA-o-methyl transferase gene (fmtD), and not jmtD.
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First purified FA-o-MT from any insect order with preferred biological activity for FA and not JHA.