Reproducibility studies for experimental epitope detection in macrophages (EDIM)
详细信息 查看全文
- 作者：Dennis Japinka ; f ; g ; dennisjapink@gmail.com" class="auth_mail ; Marius Napb ; m.nap@atriummc.nl" class="auth_mail ; Meindert N. Sosefa ; m.sosef@atriummc.nl" class="auth_mail ; Patty J. Nelemansd ; patty.nelemans@maastrichtuniversity.nl" class="auth_mail ; Johannes F. Coye ; j.coy@tavarlin.de" class="auth_mail ; Geerard Beetsf ; g.beets@maastrichtuniversity.nl" class="auth_mail ; Maarten F. von Meyenfeldtf ; mf.vonmeyenfeldt@maastrichtuniversity.nl" class="auth_mail ; Math P.G. Leersc ; m.leers@atriummc.nl" class="auth_mail
- 关键词：APC ; allophycocyanin ; CD ; cluster of differentiation ; CEA ; carcinoembryonic antigen ; CEA-IM ; CEA in macrophages ; CK ; cytokeratin ; CK-IM ; CK in macrophages ; CRC ; colorectal cancer ; EDIM ; epitope detection in macrophages ; EDTA ; ethylenediaminetetraacetic acid ; FI ; fluorescence intensity ; FITC ; fluorescein isothyocyanate ; FMO ; fluorescence-minus-one ; FSC ; forward scatter ; ICC ; intra-class correlation coefficient ; M30 ; M30 neo-epitope ; M30-IM ; M30 in macrophages ; mFMO ; modified fluorescence-minus-on
- 刊名：Journal of Immunological Methods
- 出版年：May 2014
- 年：2014
- 卷：407
- 期：Complete
- 页码：40-47
- 全文大小：736 K
文摘
- •
Common CRC blood diagnostics and monitoring lack sensitivity and specificity.
- •
The EDIM assay is a promising novel epitope detection method in macrophages for CRC.
- •
Intra- and inter-assay and transferability assessment showed reproducible results.
- •
Biological variability is interfering as usual with assay reproducibility.
- •
EDIM-assay performs technically similar to serum tumor marker assays.