Differential expression of adenylate kinase 4 in the context of disparate stress response strategies of HEK293 and HepG2 cells

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• 作者：Fanzhi Kong^a ; ¹ ; ² ; Bert Binas^a ; ¹ ; Jae Hee Moon^a ; Sung Soo Kang^a ; Hyo Joon Kim^a ; ^b ; ^{kimhj104@hanyang.ac.kr}
• 关键词：Adenylate kinase ; Hypoxia ; Cell cycle ; HepG2 cells ; HEK293 cells
• 刊名：Archives of Biochemistry and Biophysics
• 出版年：2013
• 出版时间：May, 2013
• 年：2013
• 卷：533
• 期：1-2
• 页码：11-17
• 全文大小：1155 K

文摘

Adenylate kinase isozyme 4 (AK4) belongs to a family of nucleotide monophosphate kinases involved in energy metabolism. Recently, AK4 was reported to play a role in protection from stress: In HEK293 cells, hypoxia increases AK4 expression but does not affect proliferation or viability, while RNA interference (RNAi) directed against AK4 inhibits proliferation and promotes death. By contrast, we show here that HepG2 cells showed much higher AK4 levels, which decreased under hypoxia along with markedly reduced cell proliferation and increased cell death. Nevertheless, RNAi directed against AK4 inhibited cell proliferation and caused death in both cell types, although cell cycle parameters were affected only in HepG2 cells. Hence reductions of AK4 levels were always associated with cell death. These results extend the notion of a stress-protective function of AK4 to a novel physiological context and show that AK4-mediated stress protection is not limited to one particular death scenario. Our data also allow the hypothesis that the different basal AK4 levels reflect different basal stress levels, causing alternative responses to additional stress.