Differential gene expression after total replacement of dietary fish meal and fish oil by plant products in rainbow trout (Oncorhynchus mykiss

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• 作者：S. Panserat ; G.A. Hortopan ; E. Plagnes-Juan ; C. Kolditz ; M. Lansard ; S. Skiba-Cassy ; D. Esquerré ; I. Geurden ; F. Mé ; dale ; S. Kaushik ; G. Corraze
• 关键词：Marine ingredients ; Vegetable products ; Liver ; Metabolism ; Transcriptome ; Rainbow trout
• 刊名：Aquaculture
• 出版年：2009
• 出版时间：1 September 2009
• 年：2009
• 卷：294
• 期：1-2
• 页码：123-131
• 全文大小：269 K

文摘

Our objective was to analyse the hepatic transcriptomes of juvenile rainbow trout fed with a plant-based diet. We focused our analysis on the total replacement of fish meal (FM) and fish oil (FO) by a 100 % plant-based diet (0 % FM, 0 % FO). We analysed the postprandial hepatic transcriptomes of rainbow trout fed the two diets 8 h after feeding. Six total hepatic RNAs from each dietary group were hybridised against a trout cDNA microarray (9K). After treatment of the data respecting the standard MIAME (Minimum Information About a Microarray Experiment) protocol, we found that 176 hepatic genes were differentially expressed between fish fed the two diets: 96 and 80 were over-expressed and under-expressed, respectively, in trout fed the plant-based diet. A large majority of differentially expressed genes were involved in metabolism (57 % ) and the others in cellular processes (21 % ) and transport (10 % ). Among the genes involved in metabolism (n = 86), 37 % were associated with protein metabolism (proteolysis, amino acid catabolism), 21 % with lipid metabolism (fatty acid biosynthesis, cholesterol biosynthesis), 30 % with nucleic acid metabolism and 8 % with glucose metabolism. Specifically, we found in rainbow trout fed the 100 % plant diet an over-expression of genes involved in lipid biosynthesis (cholesterol metabolism and desaturation of polyunsaturated fatty acids) and an over-expression of a new metabolic actor, i.e., glycerol kinase which plays a key role at the interface of glucose-lipid metabolism. Overall, these data demonstrate that a number of intermediary metabolic effects occur in trout fed a totally plant-based diet.