The aim of
this work was
to isola
te and cul
tiva
te a subpopula
tion of pluripo
ten
t s
tem cells presen
t in peripheral blood of differen
t animal species, frequen
tly used in labora
tory s
tudies (mice, ra
ts and hams
ters). Pluripo
ten
t s
tem cells (PSCs), already described in human beings, are fibroblas
t-like cells
tha
t exhibi
t a CD34 marker, specific for haema
topoie
tic s
tem cells. Commonly used human commercial media were inves
tiga
ted for cul
turing animal PSCs.
These findings suggest that this simple and standardized methodology may be applicable in several fields such as the study of the pharmacological effects of drugs on the haematopoietic line and the study of new strategies in cellular therapy for some human diseases.