Analysis of structural heterogeneity of fucoidan from Hizikia fusiforme by ES-CID-MS/MS
详细信息 查看全文
- 作者：Peipei Wang ; Xiaoliang Zhao ; Youjing Lv ; Yanan Liu ; Yinzhi Lang ; Ji ; ong Wu ; Xin Liu ; Miaomiao Li ; Guangli Yu
- 关键词：Fucoidan ; Oligosaccharides ; ES-CID-MS/MS ; Hizikia fusiforme
- 刊名：Carbohydrate Polymers
- 出版年：2012
- 出版时间：1 September, 2012
- 年：2012
- 卷：90
- 期：1
- 页码：602-607
- 全文大小：655 K
文摘
A fucoidan from the brown alga Hizikia fusiforme was extracted with hot water and fractionated by anion-exchange chromatography. The main fraction YF5 was depolymerized by partial acid hydrolysis and further purified by gel filtration chromatograph. The sequences of oligosaccharides generated were then determined by ES-CID-MS/MS, which gave directly information on the structural heterogeneity of fucoidan from H. fusiforme. It was shown that YF5 had a backbone of a repeating disaccharide unit of [¡ú4GlcA¦Â1,2Man¦Á1¡ú], in good agreement with previous report by NMR and methylation analysis. In the product-ion spectra, the unique 0.2A type fragmentation was important to establish the presence of a 2-linked Man in the backbone structure. In addition, abundant novel heterogeneous branched structural fragments were also detected and characterized: Fuc1,3Fuc, Fuc1,3Gal, Fuc1,4GlcA, Gal1,4Gal, Gal1,4GlcA and GlcA1,4GlcA. The sulfation mainly occurred at C2 or C4 of the fucose residue and C2, C4 or C6 of the galactose residue.