Sequence-specific Methyltransferase-Induced Labelling (SMILing) of plasmid DNA for studying cell transfection
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- 作者：Falk H.-G. Schmidt ; Michael Hü ; ben ; Basar Gider ; Franç ; ois Renault ; Marie-Paule Teulade-Fichou ; Elmar Weinhold
- 关键词：Aziridine cofactor ; Cofactor engineering ; DNA methyltransferase ; Enzymatic DNA labelling ; DNA modification ; Cell transfection ; DNA trafficking
- 刊名：Bioorganic and Medicinal Chemistry
- 出版年：2008
- 出版时间：1 January 2008
- 年：2008
- 卷：16
- 期：1
- 页码：40-48
- 全文大小：708 K
文摘
Plasmid DNA (pUC19 and pBR322) was sequence-specifically, covalently labelled with Cy3 fluorophores using a newly synthesised N-adenosylaziridine cofactor and the DNA methyltransferase M.TaqI. The fluorescently labelled plasmids were used for transfection of mammalian cells and their intracellular distribution was visualised by epifluorescence and confocal fluorescence microscopy. Although these prokaryotic plasmids do not contain nuclear import sequences, translocation into the nuclei was observed.