Fluorescent Cargo Proteins in Pancreatic β

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• 作者：Darren J. Michael ; Xuehui Geng ; Niamh X. Cawley ; Y. Peng Loh ; Christopher J. Rhodes ; Peter Drain ; Robert H. Chow
• 刊名：Biophysical Journal
• 出版年：2004
• 出版时间：December 2004
• 年：2004
• 卷：87
• 期：6
• 页码：L03-L05
• 全文大小：175 K

文摘

We compared secretion kinetics for four different fluorescent cargo proteins, each targeted to the lumen of insulin secretory vesicles. Upon stimulation, individual vesicles displayed one of four distinct patterns of fluorescence change: i), disappearance, ii), dimming, iii), transient brightening, or iv), persistent brightening. For each fusion protein, a different pattern of fluorescence change dominated. Furthermore, we demonstrated that the dominant pattern depends upon both i), the specific choice of fluorescent protein, and ii), the sequence of amino acids linking the cargo protein to the fluorescent protein. Thus, in β-cells, experiments involving fluorescent cargo proteins for the study of exocytosis must be interpreted carefully, as design of a fluorescent cargo protein determines secretion kinetics at exocytosis.