- 作者：Gilda G. Hillman ; Vinita Singh-Gupta ; David J. Hoogstra ; Lisa Abernathy ; Joseph Rakowski ; Christopher K. Yunker ; Shoshana E. Rothstein ; Fazlul H. Sarkar ; Shirish Gadgeel ; Andre A. Konski ; Fulvio Lonardo ; Michael C. Joiner
- 关键词：Lung cancer ; Radiation ; Soy isoflavones
- 刊名：Radiotherapy and Oncology
- 出版年：October, 2013
- 年：2013
- 卷：109
- 期：1
- 页码：117-125
- 全文大小：2436 K
Background
Radiotherapy of locally-advanced non-small cell lung cancer is limited by radiation-induced pneumonitis and fibrosis. We have further investigated the role of soy isoflavones to improve the effect of a high intensity radiation and reduce lung damage in a pre-clinical lung tumor model.Methods
Human A549 NSCLC cells were injected i.v. in nude mice to generate a large tumor burden in the lungs. Mice were treated with lung irradiation at 10 Gy and with oral soy. The therapy effect on the tumor cells and surrounding lung tissue was analyzed on lung sections stained with H&E, Ki-67 and Masson鈥檚 Trichrome. Pneumonitis and vascular damage were evaluated by measurements of alveolar septa and immunofluorescent staining of vessel walls.
Results
Combined soy and radiation caused a significantly stronger inhibition of tumor progression compared to each modality alone in contrast to large invasive tumor nodules seen in control mice. At the same time, soy reduced radiation injury in lung tissue by decreasing pneumonitis, fibrosis and protecting alveolar septa, bronchioles and vessels.
Conclusions
These studies demonstrate a differential effect of soy isoflavones on augmenting tumor destruction induced by radiation while radioprotecting the normal lung tissue and support using soy to alleviate radiotoxicity in lung cancer.