Biochemical characterization of the tomato phosphatidylinositol-specific phospholipase C (PI-PLC) family and its role in plant immunity
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- 作者：Ahmed M. Abd-El-Haliema ; 1 ; Jack H. Vossenb ; Arjan van Zeijla ; 2 ; Sara Dezhsetanb ; 3 ; Christa Testerinkc ; Michael F. Seidla ; Martina Beckd ; 4 ; James Struttd ; 5 ; Silke Robatzekd ; Matthieu H.A.J. Joostena ; matthieu.joosten@wur.nl" class="auth_mail" title="E-mail the corresponding author
- 关键词：At ; Arabidopsis thaliana ; DAG ; diacylglycerol ; ETI ; effector-triggered immunity ; HR ; hypersensitive response ; InsP3 ; inositol 1 ; 4 ; 5-triphosphate ; PA ; phosphatidic acid ; PAMPs ; pathogen-associated molecular patterns ; PI ; phosphatidylinositol ; PI-PLC ; phosphatidylinositol-specific phospholipase C ; PIP ; phosphatidylinositol phosphate ; PIP2 ; phosphatidylinositol-4 ; 5-bisphosphate ; PLC ; phospholipase C ; PTI ; PAMP-triggered immunity ; PKC ; protein kinase C ; Sl ; Solanum lycopersicum ; TLC ; thin layer chro
- 刊名：Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：1861
- 期：9
- 页码：1365-1378
- 全文大小：1911 K
文摘
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Seven genes encoding PLCs form tomato were identified.
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Recombinant tomato PLC proteins showed typical PLC activity.
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Tomato PLC2, PLC4 and PLC5 showed distinct requirements for Ca2 + ions and the pH.
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The activity of each tomato PLC enzyme is probably differentially regulated in vivo.
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The response to flg22 as well as FLS2 endocytosis are suppressed by inhibition of PLC activity.