Constitutive production and efficient secretion of soluble full-length streptavidin by an Escherichia coli ‘leaky mutant’
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- 作者：Jakob Michael Mü ; ller<sup> ; sup><sup>1sup><sup> ; sup><sup>jmu@fermtech.techfak.uni-bielefeld.de" class="auth_mail" title="E-mail the corresponding authorsup> ; David Wetzel ; Erwin Flaschel ; Karl Friehs ; Joe Max Risse
- 关键词：SAV ; streptavidin ; vvm ; volume per volume and minute
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：10 March 2016
- 年：2016
- 卷：221
- 期：Complete
- 页码：91-100
- 全文大小：1629 K
文摘
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Streptavidin (SAV) production by an E. coli ‘leaky mutant’ was established.
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Product secretion was improved by cultivation at 30 °C and Triton<sup>®sup> supplementation.
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bglA leader, constitutive bla promoter and full-length SAV gene were beneficial.
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A bioreactor fed-batch process led to 2.6 μM of tetrameric SAV in 40 h (65.2 nM/h).
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Less than 10% of biotin-blocked binding sites indicate a highly bioactive product.