A Role for Cdc2- and PP2A-Mediated Regulation of Emi2 in the Maintenance of CSF Arrest

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• 作者：Qiju Wu ; Yanxiang Guo ; Ayumi Yamada ; Jennifer A. Perry ; Michael Z. Wang ; Marito Araki ; Christopher D. Freel ; Jeffrey J. Tung ; Wanli Tang ; Seth S. Margolis ; Peter K. Jackson ; Hiroyuki Yamano ; Maki Asan
• 关键词：SIGNALING ; CELLCYCLE
• 刊名：Current Biology
• 出版年：2007
• 出版时间：6 February 2007
• 年：2007
• 卷：17
• 期：3
• 页码：213-224
• 全文大小：1346 K

文摘

Vertebrate oocytes are arrested in metaphase II of meiosis prior to fertilization by cytostatic factor (CSF). CSF enforces a cell-cycle arrest by inhibiting the anaphase-promoting complex (APC), an E3 ubiquitin ligase that targets Cyclin B for degradation. Although Cyclin B synthesis is ongoing during CSF arrest, constant Cyclin B levels are maintained. To achieve this, oocytes allow continuous slow Cyclin B degradation, without eliminating the bulk of Cyclin B, which would induce release from CSF arrest. However, the mechanism that controls this continuous degradation is not understood.

Results

We report here the molecular details of a negative feedback loop wherein Cyclin B promotes its own destruction through Cdc2/Cyclin B-mediated phosphorylation and inhibition of the APC inhibitor Emi2. Emi2 bound to the core APC, and this binding was disrupted by Cdc2/Cyclin B, without affecting Emi2 protein stability. Cdc2-mediated phosphorylation of Emi2 was antagonized by PP2A, which could bind to Emi2 and promote Emi2-APC interactions.

Conclusions

Constant Cyclin B levels are maintained during a CSF arrest through the regulation of Emi2 activity. A balance between Cdc2 and PP2A controls Emi2 phosphorylation, which in turn controls the ability of Emi2 to bind to and inhibit the APC. This balance allows proper maintenance of Cyclin B levels and Cdc2 kinase activity during CSF arrest.