G-quadruplex formation in the Oct4 promoter positively regulates Oct4 expression

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• 作者：Daniel Renčiuk^a ; ¹ ; Jan Ryne&scaron ; ^b ; ¹ ; Iva Kejnovská ; ^a ; Silvie Foldynová ; -Trantí ; rková ; ^b ; Michael Andä ; ng^b ; ^c ; Luká ; &scaron ; Trantí ; rek^b ; ^{lukas.trantirek@ceitec.muni.cz} ; Michaela Vorlí ; čková ; ^a ; ^mifi@ibp.cz
• 关键词：ESC ; embryonic stem cells ; CSC ; cancer stem cells ; PQS ; potential quadruplex-forming site ; NMM ; N-methyl mesoporphyrin IX
• 刊名：Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：1860
• 期：2
• 页码：175-183
• 全文大小：1157 K
• 卷排序：1860

文摘

Oct4 is a transcription factor responsible for the maintenance of pluripotency. Guanine rich region at the Oct4 transcription start forms a parallel G-quadruplex. The Oct4 expression is directly proportional to the quadruplex stability. Quadruplex-stabilizing ligand rescues inhibiting effect of destabilizing mutations. The quadruplex acts as a strong, positive regulator of Oct4 expression.