Enhancement of cytochrome c catalytic behaviour by affecting the heme environment using functionalized carbon-based nanomaterials

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• 作者：Michaela Patila ; Ioannis V. Pavlidis ; Evmorfia K. Diamanti ; Petros Katapodis ; Dimitrios Gournis ; Haralampos Stamatis
• 关键词：CBNs ; carbon-based nanomaterials ; CD ; circular dichroism ; CNT-COOH ; oxidized multi-wall carbon nanotubes ; CNT-C4-COOH and CNT-C10-COOH ; multi-wall carbon nanotubes with terminal carboxyl groups ; CNT-C6-NH2 ; multi-wall carbon nanotubes with terminal amine
• 刊名：Process Biochemistry
• 出版年：2013
• 出版时间：July, 2013
• 年：2013
• 卷：48
• 期：7
• 页码：1010-1017
• 全文大小：1892 K

文摘

The effect of carbon-based nanomaterials (CBNs), such as multi-wall carbon nanotubes (CNTs) and graphene oxide (GO) nanomaterials functionalized with carboxyl, alkyl and amine groups, on the peroxidase-like activity and structure of cytochrome c (cyt c) was investigated. The catalytic efficiency of cyt c increases up to 78-fold in the presence of graphene oxide and up to 2.5-fold in the presence of other functionalized CBNs. Moreover, the use of functionalized CBNs enhances the thermal stability of the protein as well as its tolerance against hydrogen peroxide up to 2.5-fold. UV-vis and circular dichroism spectroscopy studies suggest that the increase in the peroxidase activity of cyt c in the presence of some functionalized GO nanomaterials, correlates to perturbations of the heme microenvironment, while the secondary structure of the enzyme remains intact. These results indicate that the beneficial effect the functionalized CBNs have on the activity and on the stability of cyt c depends on CBNs geometry and surface functionalization.