Myelination and oxidative stress alterations in the cerebellum of the G72/G30 transgenic schizophrenia mouse model

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• 作者：Michaela D. Filiou ; Larysa Teplytska ; David M. Otte ; Andreas Zimmer ; Christoph W. Turck
• 关键词：Schizophrenia ; G72 ; 15N metabolic labeling ; Quantitative proteomics ; Myelination ; Oxidative stress
• 刊名：Journal of Psychiatric Research
• 出版年：2012
• 出版时间：October, 2012
• 年：2012
• 卷：46
• 期：10
• 页码：1359-1365
• 全文大小：906 K

文摘

G72/G30 is a primate-specific locus that has been repeatedly implicated as a risk factor in genetic studies of schizophrenia. The function of the longest G72 splice variant (LG72 protein) encoded by this locus is not fully understood. To investigate the role of the LG72 protein in?vivo, we have generated transgenic (G72Tg) mice carrying the G72/G30 locus that exhibit schizophrenia-like symptoms. We investigated protein expression alterations in the cerebella of G72Tg compared to wild type (WT) mice using a proteomics approach based on in?vivo ¹⁵N metabolic labeling and quantitative mass spectrometry (MS). Our data revealed expression level differences of proteins involved in myelin-related processes, oxidative stress and mitochondrial function. Furthermore, in silico pathway analyses suggested common regulators and targets for the observed protein alterations. Our work sheds light on the functional role of the LG72 protein and pinpoints molecular correlates of schizophrenia-like behavior.