¹⁵N metabolic labeling: Evidence for a stable isotope effect on plasma protein levels and peptide chromatographic retention times

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• 作者：Christian Webhofer ; Yaoyang Zhang ; Jan Brusis ; Stefan Reckow ; Rainer L ; graf ; Giuseppina Maccarrone ; Christoph W. Turck ; Michaela D. Filiou
• 关键词：C5 ; complement 5 ; FWHM ; full width at half maximum ; HAB ; high anxiety-related behavior ; Mup ; major urinary protein ; Serpina6 ; corticosteroid-binding globulin ; TPP ; trans-proteomic pipeline ; TST ; tail suspension test ; XIC ; extracted ion chromatogram
• 刊名：Journal of Proteomics
• 出版年：2013
• 出版时间：2 August, 2013
• 年：2013
• 卷：88
• 期：Complete
• 页码：27-33
• 全文大小：506 K

文摘

Many quantitative proteomics methods rely on protein and peptide labeling with stable isotopes. We have recently found that the introduction of ¹⁵N into organisms via in vivo metabolic labeling affects protein expression levels as well as metabolic pathways and behavioral phenotypes. Here, we present further evidence for a stable isotope effect based on the plasma proteome analysis of ¹⁵N-labeled mice. We compared plasma proteomes of ¹⁵N-labeled and unlabeled (¹⁴N) mice by quantitative MS. We found a number of protein level differences, some of which were verified immunochemically. In addition, we observed divergent chromatographic retention time and peak full width at half maximum (FWHM) between ¹⁵N-labeled and ¹⁴N tryptic peptides. Our data point toward a systemic effect of the introduction of heavy isotopes in vivo.

This article is part of a Special Issue entitled: New Horizons and Applications for Proteomics [EuPA 2012].