Antiarrhythmic effect of acute oxygen-ozone administration to rats

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• 作者：Clara Di Filippo ; Carmela Cervone ; Claudia Rossi ; Cristina di Ronza ; Raffaele Marfella ; Paola Capodanno ; Carlo Luongo ; Francesco Rossi ; Michele D'Amico
• 关键词：Ozone ; Arrhythmias ; Ischemia ; Reperfusion ; Aconitine ; Potassium chloride ; (Rats)
• 刊名：European Journal of Pharmacology
• 出版年：2010
• 出版时间：10 March 2010
• 年：2010
• 卷：629
• 期：1-3
• 页码：89-95
• 全文大小：848 K

文摘

The antiarrhythmic effects of 100; 150; and 300 µg/kg i.p. oxygen/ozone mixture were tested on arrhythmias induced by i) ischemia; ii) ischemia/reperfusion; iii) aconitine (15 µg/kg/i.v.); potassium chloride (1.5 % i.v.) in rats. 25 min of cardiac left descending coronary artery ischemia caused severe incidence of ventricular tachycardia, ventricular fibrillation and mortality. These were significantly reduced by pre-treatment of rats with oxygen/ozone mixture at doses of 150 and 300 µg/kg. In separate experiments using a protocol of 5 min ischemia followed by 8 min reperfusion this caused arrhythmias starting within 6 ± 1 s. The incidence of ventricular tachycardia was 100 % , while ventricular fibrillation occurred in 75 % of the animals and lasted 85 ± 14 s. The mortality was 62.5 % . These figures were significantly (P < 0.01) reduced in animals treated with 150 µg/kg oxygen/ozone and a substantial increase observed with 300 µg/kg, whilst not affected by the lower dose of 100 µg/kg.

150 and 300 µg/kg oxygen/ozone prolonged the onset time for the appearance of arrhythmias induced by aconitine (300 µg/kg oxygen/ozone,  81 % longer). Oxygen/ozone also reduced the ventricular tachycardia duration, ventricular fibrillation incidence, arrhythmia score, and increased the rat's survival rate. As for example, this latter was increased from 25 % (aconitine) to 50 % (aconitine + oxygen/ozone 150 µg/kg). 100 µg/kg oxygen/ozone was without effect. None of the oxygen/ozone doses affected the arrhythmias caused by potassium chloride 1.5 % i.v.

All the oxygen/ozone antiarrhythmic effects were similar to those observed with lidocaine (1.5 mg/kg i.v.). In conclusion, oxygen/ozone has antiarrhythmic effects against arrhythmias caused by aconitine, myocardial ischemia and ischemia/reperfusion.