Ultrasound-assisted microbubbles gene transfer in tendons for gene therapy

详细信息    查看全文

• 作者：Anthony Delal ; e ; Michel-Francis Bureau ; Patrick Midoux ; Ayache Bouakaz ; Chantal Pichon
• 关键词：Ultrasound ; Microbubbles ; Tendon ; Gene transfer ; Gene therapy
• 刊名：Ultrasonics
• 出版年：2010
• 出版时间：February 2010
• 年：2010
• 卷：50
• 期：2
• 页码：269-272
• 全文大小：213 K

文摘

Our study aimed at evaluating the use of ultrasound-assisted microbubbles gene transfer in mice Achilles tendons. Using a plasmid encoding luciferase gene, it was found that an efficient and stable gene expression for more than two weeks was obtained when tendons were injected with 10 μg of plasmid in the presence of 5 × 10⁵ BR14 microbubbles with the following acoustic parameters: 1 MHz, 200 kPa, 40 % duty cycle and 10 min of exposure time. The rate of gene expression was 100-fold higher than that obtained with naked plasmid injected alone without ultrasound or with ultrasound in absence of microbubbles. The long term expression of transgene makes ultrasound-assisted microbubble a suitable method for gene therapy in tendons.