Simultaneous gas-chromatographic measurement of rhamnose, lactulose and sucrose and their application in the testing gastrointestinal permeability

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• 作者：Abazia ; Cristiana ; Ferrara ; Renata ; Corsaro ; Maria Michela ; Barone ; Gaspare ; Coccoli ; Pietro ; et. al.
• 关键词：Gastric and intestinal permeability ; Celiac disease ; Sugars ; Urine ; GC methods
• 刊名：Clinica Chimica Acta
• 出版年：2003
• 出版时间：December, 2003
• 年：2003
• 卷：338
• 期：1-2
• 页码：25-32
• 全文大小：192 K

文摘

Background: As it is important to test gastric and intestinal permeability simultaneously in gastrointestinal disorders such as Celiac disease, we developed a gas-chromatographic (GC) method to estimate rhamnose (l-rh), lactulose (Lacl) and sucrose (Suc) in urine. Methods: The method is based on the use of alditol acetate derivatives giving a lower number of GC peaks than reducing sugars do. Acetate derivatives are more stable and less expensive than GC silylates and keep the flame-detector cleaner. We checked the chemical stability of alditol acetates by verifying the reproducibility of the standard curve of a sugar derivative sample which had been stored for 2 months at −20 °C. Results: The calibration proved linear over the range 0.1–1 μg of sugar injected. Analytical sugar recovery was 88 % ±19.4 % (mean±S.D.) for rhamnose, 105 % ±7.4 % for sucrose and 102 % ±2.4 % for lactulose. Mean within-day precision (CV) was 7.7 % for rhamnose, 5.7 % for sucrose and 1.9 % for lactulose, and between-day (CV) was 6.7 % for rhamnose, 3.9 % for sucrose and 1.6 % for lactulose. The rhamnose, lactulose and sucrose as the lactulose/rhamnose ratio clearly differentiated 25 healthy controls from 36 patients with active gluten-sensitive enteropathy. Conclusions: A fast, reliable and cheap gas-chromatographic method is presented here to evaluate gastric and intestinal permeability.