Metabolism of triglyceride-rich lipoproteins and lipid transfer to high-density lipoprotein in young obese and normal-weight patients with polycystic ovary syndrome

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• 作者：Michelle P. Rocha ; Raul C. Maranhã ; o ; Talita M. Seydell ; Cristiano R.G. Barcellos ; Edmundo C. Baracat ; Sylvia A.Y. Hayashida ; Sé ; rgio P. Bydlowski ; José ; A.M. Marcondes
• 关键词：Polycystic ovary syndrome ; insulin resistance ; triglyceride-rich lipoprotein remnants ; atherosclerosis ; transfer lipoproteins
• 刊名：Fertility and Sterility
• 出版年：2010
• 出版时间：April 2010
• 年：2010
• 卷：93
• 期：6
• 页码：1948-1956
• 全文大小：321 K

文摘

Objective

To clarify whether the metabolism of triglyceride-rich lipoproteins and lipid transfer to high-density lipoprotein (HDL) are altered in patients with polycystic ovary syndrome (PCOS).

Design

Case–control study.

Setting

Endocrinology clinics.

Patient(s)

Eight normal-weight (NW) and 15 obese (Ob) patients with PCOS were compared with 10 NW and 10 Ob women without PCOS paired for age and body mass index.

Intervention(s)

Determination of triglyceride-rich lipoprotein metabolism and lipid transfer to HDL.

Main Outcome Measure(s)

Participants were injected triglyceride-rich emulsions labeled with ¹⁴C-cholesteryl esters and ³H-triglycerides and the fractional clearance rate (FCR, in min⁻¹) of labels was determined. Lipid transfer from artificial nanoemulsions to HDL was performed by incubating radioactively labeled lipid nanoemulsions with plasma during 1 hour, followed by radioactive counting of HDL-containing supernatant after chemical precipitation.

Result(s)

Lipolysis estimated by triglyceride FCR was equal in PCOS groups (NW = 0.043 ± 0.032, Ob = 0.033 ± 0.009) and respective controls (NW = 0.039 ± 0.015, Ob = 0.044 ± 0.019). However, the remnant removal as estimated by cholesteryl ester FCR was reduced in both PCOS groups (NW = 0.005 ± 0.006, Ob = 0.005 ± 0.005) compared with controls (NW = 0.016 ± 0.006, Ob = 0.011 ± 0.072). Lipid transfer rates were not different among groups, but triglyceride transfer rates were positively correlated with homeostasis model assessment estimate of insulin resistance in PCOS.

Conclusion(s)

PCOS patients showed decreased removal of atherogenic remnants even when fasting glucose was <100 mg/dL. This reinforces the usefulness of the measures taken to prevent cardiovascular events in PCOS patients.