Sensitive Replicate Real-Time Quantitative PCR of BCR-ABL Shows Deep Molecular Responses in Long-Term Post-Allogeneic Stem Cell Transplantation Chronic Myeloid Leukemia Patients
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- 作者：Maya Koren-Michowitz1 ; 2 ; m.koren.michowitz@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Avichai Shimoni1 ; Filomena Daraio3 ; Francesca Crasto3 ; Roberta Lorenzatti3 ; Yulia Volchek1 ; Ninette Amariglio1 ; Enrico Gottardi3 ; Giuseppe Saglio3 ; Arnon Nagler1 ; 2
- 关键词：Quantitative PCR ; Stem cell transplantation ; Chronic myeloid leukemia
- 刊名：Biology of Blood and Marrow Transplantation
- 出版年：2015
- 出版时间：October 2015
- 年：2015
- 卷：21
- 期：10
- 页码：1852-1855
- 全文大小：351 K
文摘
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Patients with chronic myeloid leukemia after stem cell transplantation were studied with a sensitive quantitative PCR
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Replicate real-time quantitative PCR uses RT-qPCR methods with 82 replicates per sample
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Replicate real-time quantitative PCR increased assay sensitivity to ≥ 10−5.5 in 95% of studied cases
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At 4 years after stem cell transplantation, all tested patients were negative for BCR-ABL including 5 in accelerated phase