Epitope mapping of Campylobacter jejuni flagellar capping protein (FliD) by chicken (Gallus gallus domesticus) sera
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- 作者：Hung-Yueh Yeha ; hungyueh.yeh@ars.usda.gov" class="auth_mail" title="E-mail the corresponding author ; Arife Ezgi Tellib ; Jarra F. Jagnec ; Christopher L. Benson IId ; Kelli L. Hietta ; John E. Linea
- 关键词：Campylobacter jejuni ; Flagellar capping protein ; FliD ; Epitope mapping ; Zoonosis ; Foodborne pathogen
- 刊名：Comparative Immunology, Microbiology and Infectious Diseases
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：49
- 期：Complete
- 页码：76-81
- 全文大小：623 K
文摘
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A set of 158 synthetic peptides of 15-mer overlapping with 11 amino acid residues of FliD on peptide microarrays was mapped with field chicken sera.
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Peptides 24, 91 and 92 had relatively high reactivity to 64 individual sera, indicating these peptides represented the shared epitopes on FliD.
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Peptides 24, 91 and 92 were also recognized by sera from chickens immunized with the purified recombinant FliD protein.