Development of effective QCM biosensors by cyclopropylamine plasma polymerization and antibody immobilization using cross-linking reactions
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- 作者：E. Makhnevaa ; b ; A. Manakhova ; P. Sklá ; dalc ; d ; L. Zají ; čková ; a ; b ; lenkaz@physics.muni.cz" class="auth_mail" title="E-mail the corresponding author
- 关键词：Plasma polymerization ; Cyclopropylamine ; Stable amine-rich films ; Quartz crystal microbalance ; Immunosensor ; Label-free detection
- 刊名：Surface and Coatings Technology
- 出版年：2016
- 出版时间：25 March 2016
- 年：2016
- 卷：290
- 期：Complete
- 页码：116-123
- 全文大小：987 K
文摘
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Plasma polymerized cyclopropylamine can be used in effective immunosensors.
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Films with similar amount of NH2 but different stability show different performance.
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Three different antibody immobilization methods were explored.
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GA and sulfo-SMCC methods provide stable baseline, selective and high response.