Piezometric biosensors for anti-apoptotic protein survivin based on buried positive-potential barrier and immobilized monoclonal antibodies
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- 作者：Magdalena Stobieckaa ; magdalena_stobiecka@sggw.pl" class="auth_mail" title="E-mail the corresponding author ; ; Agata Chalupab ; Beata Dworakowskaa
- 关键词：Piezoimmunosensor ; Protein survivin ; Monoclonal anti-survivin antibody ; Quartz crystal microbalance ; Immunosensor
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 October 2016
- 年：2016
- 卷：84
- 期：Complete
- 页码：37-43
- 全文大小：1227 K
文摘
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New piezometric and voltammetric immunosensors for the detection of protein survivin (Sur) have been developed.
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The proposed sensors are based on positive potential barrier self-assembled monolayer and covalently bonded monoclonal anti-survivin antibody.
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A low limit of detection of 1.7 nM Sur (S/N=3σ) has been achieved.
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The piezoimmunosensor discriminates well against nonspecific interactions with UM-87 MG cell lysate components, providing high measurement specificity for Sur.