Highly regioselective hydroxylation of polydatin, a resveratrol glucoside, for one-step synthesis of astringin, a piceatannol glucoside, by P450 BM3

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• 作者：Thien-Kim Le^a ; Hyun-Hee Jang^a ; Ha Thi Huong Nguyen^a ; Tiep Thi My Doan^a ; Ga-Young Lee^a ; Ki Deok Park^b ; Taeho Ahn^c ; Young Hee Joung^a ; Hyung-Sik Kang^a ; Chul-Ho Yun^a ; ^{chyun@jnu.ac.kr} ; ^{chyun49@gmail.com}
• 关键词：NADPH ; β-nicotinamide adenine dinucleotide phosphate (reduced form) ; p-NP ; p-nitrophenol ; P450 or CYP ; cytochromes P450 ; TTN ; total turnover number ; WT ; wild-type
• 刊名：Enzyme and Microbial Technology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：97
• 期：Complete
• 页码：34-42
• 全文大小：1331 K
• 卷排序：97

文摘

A set of CYP102A1 mutants can catalyze regioselective hydroxylation of polydatin. Major product of polydatin hydroxylation is astringin, a piceatannol glucoside. An efficient enzymatic synthesis of astringin, a piceatannol glucoside, was developed.