Establishing a synergetic carbon utilization mechanism for non-catabolic use of glucose in microbial synthesis of trehalose

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• 作者：Yifei Wu^a ; ¹ ; Xinxiao Sun^a ; ¹ ; Yuheng Lin^b ; Xiaolin Shen^a ; Yaping Yang^b ; Rachit Jain^b ; Qipeng Yuan^a ; ^{yuanqp@mail.buct.edu.cn} ; Yajun Yan^b ; ^{yajunyan@uga.edu}
• 关键词：Carbon utilization ; Trehalose ; UDP-glucose ; Glycosylation ; Polysaccharide
• 刊名：Metabolic Engineering
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：39
• 期：Complete
• 页码：1-8
• 全文大小：672 K
• 卷排序：39

文摘

A synergetic carbon utilization mechanism (SynCar) is designed. A glucose-based trehalose biosynthesis model is constructed for demonstration. The enhancement on trehalose titer (131%) and yield (200%) is achieved. The highest titer of trehalose in shake flasks is 8.2 g/L.