Agaricus bisporus was effective for PAH biodegradation in multi-polluted soil.
Spent A. bisporus substrate application is the key to achieving maximum PAH removal.
The increment of available Pb did not decrease the PAH biodegradation by A. bisporus.
Sterilized SAS increased Pb availability and inhibited PAH removal by soil bacteria.
The increment of Pb availability by SAS or SSAS was very low and decreased with time.