An effective pre-treatment method for the determination of short-chain fatty acids in a complex matrix by derivatization coupled with headspace single-drop microextraction

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• 作者：Yuan Chen ; Yun Li ; Yongqiang Xiong ; Chenchen Fang ; Xiaotao Wang
• 关键词：Derivatization ; Headspace single-drop extraction ; Short-chain fatty acids ; Fatty acid ethyl esters
• 刊名：Journal of Chromatography A
• 出版年：17 January, 2014
• 年：2014
• 卷：1325
• 期：Complete
• 页码：49-55
• 全文大小：444 K

文摘

We have developed a sample preparation method involving derivatization combined with headspace single-drop microextraction (HS-SDME) for the determination of short-chain fatty acids (SCFAs) in complex matrices. The derivatization of SCFAs was conducted using the BF3/ethanol method prior to HS-SDME. The HS-SDME extraction conditions for the derivatization products (ethyl esters) of SCFAs were optimized using 1.0 渭L of dibutylphthalate (DBP), 1000 rpm stirring speed, 30% (w/v) NaCl, 20 min extraction time, and 7 mL of sample solution in a 12 mL vial. Quantitative determination of ethyl esters was performed using gas chromatography (GC). Linear calibration curves and excellent reproducibility were obtained using these optimized extraction conditions. Compared with our previous work, the significantly lower detection limits (0.11, 0.017, 0.0060, and 0.0024 渭g/mL for C₂ to C₅ SCFAs, respectively) indicate that this new method is suitable for quantitative analysis of SCFAs in complex matrices, such as the RuO4 oxidation products of kerogen or asphaltene.