Rapid extraction and determination of amphetamines in human urine samples using dispersive liquid-liquid microextraction and solidification of floating organic drop followed by high performance liquid chromatography

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• 作者：Toraj Ahmadi-Jouibari^a ; Nazir Fattahi^b ; ^{nazirfatahi@yahoo.com" class="auth_mail} ; ^{nazirfatahi@gmail.com" class="auth_mail} ; Mojtaba Shamsipur^c
• 关键词：Amphetamines ; Dispersive liquid&ndash ; liquid microextraction ; Solidification of floating organic drop ; Urine analysis
• 刊名：Journal of Pharmaceutical and Biomedical Analysis
• 出版年：June, 2014
• 年：2014
• 卷：94
• 期：Complete
• 页码：145-151
• 全文大小：1218 K

文摘

A novel, rapid, simple and sensitive dispersive liquid-liquid microextraction method based on the solidification of floating organic drop (DLLME-SFO) combined with high-performance liquid chromatography-ultraviolet detection (HPLC-UV) was used to determine amphetamine and methamphetamine in urine samples. The factors affecting the extraction efficiency of DLLME-SFO such as the kind and volume of the extraction and the disperser solvents, effect of concentration of K₂CO₃ and extraction time were investigated and the optimal extraction conditions were established. Under the optimum conditions (extraction solvent: 30.0 渭l 1-undecanol; disperser solvent: 300 渭l acetonitrile; buffer concentration: 2% (w/v) K₂CO₃ and extraction time: 1 min), calibration curves are linear in the range of 10-3000 渭g l^鈭? and limit of detections (LODs) are in the range of 2-8 渭g l^鈭?. The relative standard deviations (RSDs) for 100 渭g l^鈭? of amphetamine and methamphetamine in diluted urine are in the range of 6.2-7.8% (n = 7). The method was successfully applied for the determination of amphetamine and methamphetamine in the actual urine samples. The relative recoveries of urine samples spiked with amphetamine and methamphetamine are 87.8-113.2%. The obtained results show that DLLME-SFO combined with HPLC-UV is a fast and simple method for the determination of amphetamine and methamphetamine in urine.