Advances in digital polymerase chain reaction (dPCR) and its emerging biomedical applications

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• 作者：Lei Cao^a ; ^b ; Xingye Cui^a ; ^b ; Jie Hu^a ; ^b ; Zedong Li^a ; ^b ; Jane Ru Choi^a ; ^b ; Qingzhen Yang^a ; ^b ; Min Lin^a ; ^b ; Li Ying Hui^c ; Feng Xu^a ; ^b ; ^{fengxu@mail.xjtu.edu.cn}
• 关键词：Deoxyribonucleic acid ; Absolute quantification ; Digital PCR ; Droplet ; Microwell ; Microfluidics ; Beads
• 刊名：Biosensors and Bioelectronics
• 出版年：2017
• 出版时间：15 April 2017
• 年：2017
• 卷：90
• 期：Complete
• 页码：459-474
• 全文大小：2087 K
• 卷排序：90

文摘

Principles of all key steps of digital PCR is discussed. Conclude and compare up-to-date digital PCR strategies. Different biomedical applications of digital PCR are summarized. The existing challenges and future perspectives for digital PCR are proposed.