Analytical detection techniques for droplet microfluidics¡ªA review

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• 作者：Ying Zhu ; Qun Fang
• 关键词：Droplet-based microfluidics ; Analytical detection technique ; Imaging ; Molecular spectroscopy ; Mass spectrometry ; Capillary electrophoresis
• 刊名：Analytica Chimica Acta
• 出版年：2013
• 出版时间：17 July, 2013
• 年：2013
• 卷：787
• 期：Complete
• 页码：24-35
• 全文大小：2985 K

文摘

In the last decade, droplet-based microfluidics has undergone rapid progress in the fields of single-cell analysis, digital PCR, protein crystallization and high throughput screening. It has been proved to be a promising platform for performing chemical and biological experiments with ultra-small volumes (picoliter to nanoliter) and ultra-high throughput. The ability to analyze the content in droplet qualitatively and quantitatively is playing an increasing role in the development and application of droplet-based microfluidic systems. In this review, we summarized the analytical detection techniques used in droplet systems and discussed the advantage and disadvantage of each technique through its application. The analytical techniques mentioned in this paper include bright-field microscopy, fluorescence microscopy, laser induced fluorescence, Raman spectroscopy, electrochemistry, capillary electrophoresis, mass spectrometry, nuclear magnetic resonance spectroscopy, absorption detection, chemiluminescence, and sample pretreatment techniques. The importance of analytical detection techniques in enabling new applications is highlighted. We also discuss the future development direction of analytical detection techniques for droplet-based microfluidic systems.