Data on the inhibition of RNase inhibitor activity by a monoclonal antibody as assessed by microfluidics-based RNA electrophoresis

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• 作者：Xiao Wang ; Belete Teferedegne¹ ; Kenneth Shatzkes² ; Wei Tu ; Haruhiko Murata ; ^{haruhiko.murata@fda.hhs.gov}
• 关键词：RNA ; RNase ; RNase inhibitor ; Monoclonal antibody ; Dithiothreitol
• 刊名：Data in Brief
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：9
• 期：Complete
• 页码：417-421
• 全文大小：722 K
• 卷排序：9

文摘

Using purified reaction components, a commercial monoclonal antibody (Ab) specific to RNase inhibitor (RI) was found to interfere with the activity of RI. Total RNA was mixed with a monoclonal Ab specific to either RI (clone 3F11) or glyceraldehyde-3-phosphate dehydrogenase (GAPDH), RNase A, RI, or a combination of the above. Following incubation for 1 h at 22 °C or 37 °C, RNA integrity of the mixtures was assessed using microfluidics-based Bio-Rad Experion RNA electrophoresis. The addition of Ab 3F11 prevented RI from effectively inhibiting RNase A and therefore resulted in extensive RNA degradation. The data presented are associated with the research article entitled “Endogenous RNase Inhibitor Contributes to Stability of RNA in Crude Cell Lysates: Applicability to Reverse Transcription Quantitative PCR (RT-qPCR)” (Wang et al., 2016) [1].