Transcriptomic profiling of Arabidopsis gene expression in response to varying micronutrient zinc supply

详细信息    查看全文

• 作者：Herlâ ; nder Azevedo^a ; Sarah Gaspar Azinheiro^a ; Antonio Muñ ; oz-Mé ; rida^a ; Pedro Humberto Castro^b ; Bruno Huettel^c ; Mark G.M. Aarts^d ; Ana G.L. Assunç ; ã ; o^a ; ^b ; ^{agla@plen.ku.dk" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Microarray ; Micronutrient ; Zinc deficiency ; Arabidopsis ; bZIP
• 刊名：Genomics Data
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：7
• 期：Complete
• 页码：256-258
• 全文大小：479 K

文摘

Deficiency of the micronutrient zinc is a widespread condition in agricultural soils, causing a negative impact on crop quality and yield. Nevertheless, there is an insufficient knowledge on the regulatory and molecular mechanisms underlying the plant response to inadequate zinc nutrition [1]. This information should contribute to the development of plant-based solutions with improved nutrient-use-efficiency traits in crops. Previously, the transcription factors bZIP19 and bZIP23 were identified as essential regulators of the response to zinc deficiency in Arabidopsis thaliana [2]. A microarray experiment comparing gene expression between roots of wild-type and the mutant bzip19 bzip23, exposed to zinc deficiency, led to the identification of differentially expressed genes related with zinc homeostasis, namely its transport and plant internal translocation [2]. Here, we provide the detailed methodology, bioinformatics analysis and quality controls related to the microarray gene expression profiling published by Assunção and co-workers [2]. Most significantly, the present dataset comprises new experimental variables, including analysis of shoot tissue, and zinc sufficiency and excess supply. Thus, it expands from 8 to 42 microarrays hybridizations, which have been deposited at the Gene Expression Omnibus (GEO) under the accession number GSE77286. Overall, it provides a resource for research on the molecular basis and regulatory events of the plant response to zinc supply, emphasizing the importance of Arabidopsis bZIP19 and bZIP23 transcription factors.