Microwave-induced production of boron-doped HAp (B-HAp) and B-HAp coated composite scaffolds

详细信息    查看全文

• 作者：Ekin Ö ; . Tunç ; ay^a ; T. Tolga Demirtaş^a ; Menemşe Gü ; mü ; şderelioğlu^a ; ^b ; ^{menemse@gmail.com}
• 关键词：Boron ; Simulated body fluid ; Microwave ; Hydroxyapatite ; Chitosan ; Bone tissue engineering ; Boron doped hydroxyapatite
• 刊名：Journal of Trace Elements in Medicine and Biology
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：40
• 期：Complete
• 页码：72-81
• 全文大小：3023 K
• 卷排序：40

文摘

The aim of the present study is to produce boron (B) doped hydroxyapatite (B-HAp), which has an osteoinductive property, and investigate in-vitro osteogenesis potential of B-HAp coated chitosan (B-HAp/Ch) scaffolds. At first, B-HAp was produced by the interaction of ions within the concentrated synthetic body fluid containing boron (B-SBF) with microwave energy. Boron incorporation into HAp structure was performed by the substitution of borate ions with phosphate and hydroxyl ions. Experiments were carried out with different microwave powers and exposure times, and optimum conditions for the production of B-HAp were determined. B-HAp precipitated from B-SBF by 600 W microwave power has 1.15 ± 0.11% (w/w) B, 1.40 (w/w) Ca/P ratio, 4.30 ± 0.07% (w/w) carbonate content, 30 ± 4 nm rod-like morphology and bone-like amorphous structure. Then, chitosan scaffolds that were prepared by freeze-drying were coated with B-HAp by performing microwave-assisted precipitation in the presence of scaffolds to improve their bioactivities and mechanical properties. The formation of apatite layer and the penetration of apatites into the pores were observed by scanning electron microscopy (SEM). Fourier Transform Infrared spectroscopy (ATR-FTIR) and X-ray diffraction (XRD) analysis also confirmed the presence of B-HAp layer. As control, hydroxyapatite coated chitosan scaffolds (HAp/Ch) produced at the same conditions were used. The results of cell culture studies indicated that B releasing from scaffolds enhances proliferation and osteoblastic differentiation of MC3T3-E1 cells. This work emphasized the importance of the use of B within the scaffolds for enhancing in-vitro bone tissue engineering applications.