Co-culture model consisting of human brain microvascular endothelial and peripheral blood mononuclear cells
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- 作者：Marianne Strazzaa ; ms996@drexel.edu" class="auth_mail" title="E-mail the corresponding author ; Monique E. Mauberta ; mem446@drexel.edu" class="auth_mail" title="E-mail the corresponding author ; Vanessa Pirronea ; vpirrone@drexelmed.edu" class="auth_mail" title="E-mail the corresponding author ; Brian Wigdahla ; b ; bwigdahl@drexelmed.edu" class="auth_mail" title="E-mail the corresponding author ; Michael R. Nonnemachera ; mnonnema@drexelmed.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：BBB ; blood&ndash ; brain barrier ; BMEC ; brain microvascular endothelial cells ; CNS ; central nervous system ; FITC-D ; fluorescein isothiocyanate dextran ; PBMC ; peripheral blood mononuclear cells ; Pe ; permeability coefficient ; PTFE ; polytetrafluoroethylene ; TEER ; transendothelial electrical resistance
- 刊名：Journal of Neuroscience Methods
- 出版年：2016
- 出版时间：30 August 2016
- 年：2016
- 卷：269
- 期：Complete
- 页码：39-45
- 全文大小：1520 K
文摘
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The immortalized human BMEC cell line, hCMEC/D3, in vitro blood-brain barrier model is useful in long-term functional studies.
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hCMEC/D3 media components EBM-2 and hydrocortisone are cytotoxic to peripheral blood mononuclear cells (PBMCs).
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Co-culture media excluding EBM-2 and hydrocortisone support hCMEC/D3 barrier function as well as continued PBMC viability.
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Co-culture media can be used in assays of PBMC transmigration.