A systematic approach to stabilizing EBA-175 RII-NG for use as a malaria vaccine

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• 作者：Laura J. Peek ; Duane T. Brandau ; LaToya S. Jones ; Sangeeta B. Joshi and C. Russell Middaugh
• 关键词：Malaria ; Stability ; Protein
• 刊名：Vaccine
• 出版年：2006
• 出版时间：26 July 2006
• 年：2006
• 卷：24
• 期：31-32
• 页码：5839-5851
• 全文大小：484 K

文摘

Region II of the erythrocyte-binding antigen (EBA-175 RII) has been identified as a promising target for a malaria vaccine. A systematic approach to identify optimal preformulation conditions of a non-glycosylated (NG) antigen, EBA-175 RII-NG, has been developed. This approach consists of development of an empirical temperature/pH phase diagram, high throughput stabilizer screening and aluminum salt adjuvant adsorption studies. Using these physical methods, we developed a stable formulation for EBA-175 RII-NG at pH 6.0 with sucrose and Brij^® 35 as stabilizers and Adju-Phos^® as an adjuvant. This approach should be generally applicable to guiding the development of stable vaccine formulations.