Study of CRP immobilization on nanostructured silicon

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• 作者：Monica Simion ; Lavinia L. Ruta ; Mihaela Matache ; Irina Kleps ; Mihaela Miu ; Codruta C. Paraschivescu ; Adina Bragaru ; Teodora Ignat
• 关键词：Protein microarray ; Porous silicon ; C-reactive protein
• 刊名：Materials Science and Engineering: B
• 出版年：2010
• 出版时间：25 May 2010
• 年：2010
• 卷：169
• 期：1-3
• 页码：67-72
• 全文大小：2139 K

文摘

C-reactive protein (CRP) is a phylogenetically highly conserved plasma protein, which is widely used as an indicator of inflammatory states due to rapid increase of its plasma concentration up to 1000 times compared to normal values. Detection of CRP levels in a rapid, simultaneous and multiplex format is therefore of great interest for diagnostics. Microarray technology could provide such a multiplex format of CRP levels detection. Different nanostructured porous silicon (PS) surfaces were obtained and used for the immobilization of CRP and anti-human CRP antibodies in order to achieve an optimum microarray assay. Comparative analysis of the attachment degree and preservation of the biomolecules activity on the silicon surfaces and functionalized glass slides is also described.