Detection of Mycobacterium avium subsp. paratuberculosis in bovine milk from the state of Pernambuco, Brazil

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• 作者：Pedro Paulo Feitosa de Albuquerque ; ^{ppfalbuquerque@gmail.com} ; André ; de Souza Santos ; Orestes Luiz de Souza Neto ; Pomy de Cá ; ssia Peixoto Kim ; Erika Fernanda Torres Samico Fernandes Cavalcanti ; Jú ; nior Má ; rio Baltazar de Oliveira ; Rinaldo Aparecido Mota ; José ; Wilton Pinheiro Jú ; nior
• 关键词：Molecular biology ; Diagnosis ; Paratuberculosis ; Milk
• 刊名：Brazilian Journal of Microbiology
• 出版年：2017
• 出版时间：January-March 2017
• 年：2017
• 卷：48
• 期：1
• 页码：113-117
• 全文大小：674 K
• 卷排序：48

文摘

The aim of this study was to detect the IS900 region of Mycobacterium avium subsp. paratuberculosis (MAP) in bovine milk samples using real-time polymerase chain reaction (qPCR) and conventional PCR, and to study the agreement between these tests. A total of 121 bovine milk samples were collected from herds considered positive for MAP, from the State of Pernambuco, Brazil. MAP DNA was detected in 20 samples (16.5%) using conventional PCR and in 34 samples (28.1%) using qPCR. MAP DNA was detected in all of the 6 animal farms studied. Moderate agreement was found between qPCR and conventional PCR results, where the sensitivity and specificity of conventional PCR in relation to qPCR were 50% and 96.6%, respectively. Thus, the IS900 region of MAP was found in bovine milk samples from the State of Pernambuco. To the best of our knowledge, this is the first report of MAP DNA found in bovine milk in Northeast Brazil. We also demonstrated the qPCR technique is more sensitive than conventional PCR with respect to detection of MAP in milk samples.