Oncospheral peptide-based ELISAs as potential seroepidemiological tools for Taenia solium cysticercosis/neurocysticercosis in Venezuela

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• 作者：Elizabeth Ferrer ; M. Milagros Cort&#xe9 ; z ; Zully Cabrera ; Glenda Rojas ; Iris D&#xe1 ; vila ; Belkis Alarcó ; n de Noya ; Hilda A. P&#xe9 ; rez ; Isis Fern ; ez ; Haidee Urdaneta Romero ; Leslie J.S. Harrison
• 关键词：Cysticercosis ; Taenia saginata ; Taenia solium ; Synthetic peptides ; Antibody ELISA ; Venezuela
• 刊名：Transactions of the Royal Society of Tropical Medicine and Hygiene
• 出版年：2005
• 出版时间：August 2005
• 年：2005
• 卷：99
• 期：8
• 页码：568-576
• 全文大小：196 K

文摘

This study evaluates five synthetic peptides derived from four, potentially protective, Taenia saginata oncosphere molecules for the serodiagnosis of T. solium cysticercosis/neurocysticercosis in three distinct Venezuelan endemic regions. The peptides, all of which have been described previously, are designated HP6-3, Ts45W-1, Ts45W-5, Ts45S-10 and TEG-1. In clinically verified and seropositive hospital cases, combining the results of three of the individual peptide-based ELISAs (HP6-3, Ts45W-1 and Ts45W-5) afforded the best balance between sensitivity (85 % ) and specificity (83.5 % ), a significant improvement on the 63.6 % specificity obtained with the routinely employed T. solium cyst-fluid-based ELISA. Similarly, in the seropositive Venezuelan endemic zone samples, 89.09 % of Amerindians, 77.27 % of symptomatic rural subjects and 67.83 % of non-symptomatic rural subjects were also classed as seropositive by the combined peptide-based ELISAs. The profile of antibody recognition to individual peptides varied between the different groups of samples examined. The relevance of the above findings for the serology and prognosis of T. solium cysticercosis/neurocysticercosis in hospital- and field-based situations is discussed.