Aspergillus vertebral osteomyelitis in chronic leukocyte leukemia patient diagnosed by a novel panfungal polymerase chain reaction method

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• 作者：Lior Dayan ; Hannah Sprecher ; Amos Hananni ; Hana Rosenbaum ; Victor Milloul ; Ilana Oren
• 关键词：Polymerase chain reaction ; Osteomyelitis ; Aspergillosis ; Spine
• 刊名：The Spine Journal
• 出版年：2007
• 出版时间：September-October 2007
• 年：2007
• 卷：7
• 期：5
• 页码：615-617
• 全文大小：327 K

文摘

Vertebral osteomyelitis and disciitis caused by Aspergillus spp is a rare event. Early diagnosis and early antifungal therapy are critical in improving the prognosis for these patients. The diagnosis of invasive fungal infections is, in many cases, not straightforward and requires invasive procedures so that histological examination and culture can be performed. Furthermore, current traditional microbiological tests (ie, cultures and stains) lack the sensitivity for diagnosis of invasive aspergillosis.

Purpose

To present a case of vertebral osteomyelitis caused by Aspergillus spp diagnosed using a novel polymerase chain reaction (PCR) assay.

Study design

Case report.

Methods

Aspergillus DNA was detected in DNA extracted from the necrotic bone tissue by using a “panfungal” PCR novel method.

Results

Treatment with voriconazole was started based on the diagnosis.

conclusion

Using this novel technique enabled us to diagnose accurately an unusual bone pathogen that requires a unique treatment.