By the characterization of a rice plant hyper-susceptible to Mgnaporthe oryzae, we revealed features of symptoms in a hyper-susceptible rice plant and suppression of defense responses in the hyper-susceptible rice plant.

Features of host cellular dynamics during the biotrophic invasion of M. oryzae were analyzed. Live-cell imaging revealed that invasive hyphae are closely surrounded by host vacuolar membranes.

A long-term fluorescence imaging method for the biotrophic invasion process has been developed and clarified the timing and the way of vacuole disruption in the invaded host cells.

High-resolution imaging of the biotrophic interfacial complex (BIC) revealed that the BIC comprises aggregated host membranes intermingling with the cytosol, symplastic effectors enclosed by membranes, and the BIC base.