Lipid composition of the main fractions of rabbit semen
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- 作者：Cesare Castellini ; Raffaella Cardinali ; Alessandro Dal Bosco ; Alba Minelli and Oliva Camici
- 关键词：Rabbit spermatozoa ; Semen ; Lipid profile
- 刊名：Theriogenology
- 出版年：2006
- 出版时间：1 March 2006
- 年：2006
- 卷：65
- 期：4
- 页码：703-712
- 全文大小：155 K
文摘
Rabbit semen contains mature spermatozoa and several other fractions (seminal plasma, droplets and vesicles) which are separated by various procedures. These fractions have a variable lipid profile: spermatozoa contain the largest amount of phospholipids (PL), whereas seminal plasma, droplets and vesicles accounted for 49.8 % of the total PLs. The cholesterol content in raw semen was 811 μg/109 but was only 21–23 % in spermatozoa. The main PL classes of rabbit spermatozoa were PC, LPC, PE, PS, SM and PI, which varied according to the separation procedures used. Percoll-separated spermatozoa (Spp) showed an increase of LPC, a higher LPC/PC ratio but a lower lipid content compared to the theoretical amount. This membrane modification did not affect the number of live cells but greatly influenced the functional properties of the rabbit spermatozoa, i.e. the HOS-test and induced acrosome reaction. PC, followed by PE and LPC were the most abundant PL classes of seminal plasma, droplets and vesicles. These fractions have higher PE and SM levels and lower PC/PE + PC ratios than in the germinal cells. Some physiological implications are discussed.