Characterization of the recombinant porcine pancreas phospholipase A2 expressed in Pichia pastoris GS115 and its application to synthesis of 2-DHA-PS
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- 作者：Yihan Liua ; b ; c ; d ; Lin Huanga ; b ; c ; d ; Mingjie Lia ; b ; c ; d ; Hao Liua ; b ; c ; d ; Wei Guoa ; b ; c ; d ; Shuang Guia ; b ; c ; d ; Jinlin Niua ; b ; c ; d ; Fuping Lua ; b ; c ; d ; lfp@tust.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Porcine pancreas phospholipase A2 ; Pichia pastoris ; Recombinant protein expression ; 2-DHA-PS ; Synthesis
- 刊名：Process Biochemistry
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：51
- 期：10
- 页码：1472-1478
- 全文大小：883 K
文摘
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A novel strategy for synthesis of 1-acyl-2-docosahexaenoyl-phosphatidylserine (2-DHA-PS) using recombinant porcine pancreas phospholipase A2 (rPLA2M) is studied.
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rPLA2M is successfully expressed in Pichia pastoris GS115.
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rPLA2M displays the same activity as the native porcine pancreas phospholipase A2.
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rPLA2M is successfully applied in the synthesis of 2-DHA-PS.
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The yield of 2-DHA-PS using the rPLA2M is 23% in a safe method.