Flagellin and F4 fimbriae have opposite effects on biofilm formation and quorum sensing in F4ac+ enterotoxigenic Escherichia coli

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• 作者：Mingxu Zhou ; Zhiyan Guo ; Yang Yang ; Qiangde Duan ; Qi Zhang ; Fenghua Yao ; Jun Zhu ; Xinjun Zhang ; Philip聽R. Hardwidge ; Guoqiang Zhu
• 关键词：Flagellin ; F4 fimbriae ; Biofilm formation ; Quorum sensing
• 刊名：Veterinary Microbiology
• 出版年：10 January, 2014
• 年：2014
• 卷：168
• 期：1
• 页码：148-153
• 全文大小：624 K

文摘

Bacteria that form biofilms are often highly resistant to antibiotics and are capable of evading the host immune system. To evaluate the role of flagellin and F4 fimbriae on biofilm formation by enterotoxigenic Escherichia coli (ETEC), we deleted the fliC (encoding the major flagellin protein) and/or the faeG (encoding the major subunit of F4 fimbriae) genes from ETEC C83902. Biofilm formation was reduced in the fliC mutant but increased in the faeG mutant, as compared with the wild-type strain. The expression of AI-2 quorum sensing associated genes was regulated in the fliC and faeG mutants, consistent with the biofilm formation of these strains. But, deleting fliC and/or faeG also inhibited AI-2 quorum sensing activity.