Biosynthesis and characterization of a non-repetitive polypeptide derived from silk fibroin heavy chain
详细信息 查看全文
- 作者：Gaoqiang Yang ; Mingyang Wu ; Honggen Yi ; Jiannan Wang ; wangjn@suda.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：IPTG ; isopropyl-β-d-thiogalactoside ; SDS ; sodium dodecyl sulfate ; PAGE ; polyacrylamide gel electrophoresis ; GST ; glutathione S-transferase ; f(4) ; four repeats of designed gene motif f(1) ; f(8) ; eight repeats of designed gene motif f(1) ; F(1) ; expression product of f(1) ; F(4) ; expression product of f(4) ; F(8) ; expression product of f(8) ; GST-F(1) ; expression product of recombinant combining with GST and F(1) ; GST-F(4) ; expression product of recombinant combining with GST and F(4) ; GST-F(8) ; expre
- 刊名：Materials Science & Engineering C
- 出版年：2016
- 出版时间：1 February 2016
- 年：2016
- 卷：59
- 期：Complete
- 页码：278-285
- 全文大小：1222 K
文摘
- •
A non-repetitive domain and its multimers of silk fibroin were expressed by E. coli.
- •
The corresponding target polypeptides F(1), F(4) and F(8) were cleaved clearly.
- •
Their molecular weights are consistent with the predicted values.
- •
Their amino acid compositions are similar to the predicted sequences.
- •
The target polypeptides exhibit good hydrophilia and a negative ζ-potential.